Composite
Part:BBa_K3733044:Design
Designed by: Zhenhao Han Group: iGEM21_HZAU-China (2021-10-01)
Toxin/antitoxin HepT/MntA suicide system working at low temperatures
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This is a composite part including the RNA thermometer(hot-activate),the HepT toxin and the MntA antitoxin.
Source
Used a strong RBS to produce high levels of expression of the HepT protein and MntA protein.